Method for the Fermentative Production of L-Amino Acids with the Aid of Coryneform Bacteria Capable of Using Glycerin as the Only Carbon Source

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This invention describes a fermentation method for producing L-amino acids, such as L-lysine and L-tryptophan, using genetically engineered coryneform bacteria (particularly Corynebacterium glutamicum) that are capable of utilizing glycerin (glycerol) as the sole carbon source. By introducing and expressing specific genes involved in glycerol metabolism from other organisms (e.g., E. coli) into these bacteria—and optionally reinforcing genes in the amino acid biosynthesis pathway or weakening competing metabolic pathways—the bacteria can efficiently convert inexpensive, abundant glycerin into valuable L-amino acids for industrial purposes.

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• Biotechnological production of L-amino acids (especially L-lysine and L-tryptophan) for use as nutritional supplements in animal feed and human food.
• Manufacturing of pharmaceuticals and cosmetics that require specific amino acids as ingredients.
• Recycling and value-adding of crude glycerin waste from biodiesel production by converting it into higher-value chemicals (L-amino acids).
• Supporting sustainable and cost-effective fermentation processes in the biochemical industry by using alternative, low-cost feedstocks.
• Production of amino acids for use in microbiological growth media, agriculture, and chemicals.

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• Enables the use of cheap and abundant glycerin—a byproduct of biodiesel manufacture—as the only carbon source, reducing raw material costs compared to traditional sugars like glucose or sucrose.
• Promotes sustainable bioprocessing by utilizing industrial waste streams.
• Improves yield and efficiency in L-amino acid production due to genetic reinforcement of desired biosynthetic pathways and suppression of undesired ones.
• Extends the flexibility and industrial value of Corynebacterium glutamicum strains by allowing them to grow on non-traditional substrates.
• Supports cost-effective and eco-friendly manufacturing with reduced environmental impact.

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Patent Abstract

The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (> 0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product. In said method, bacteria are used in which other genes of the biosynthesis pathway of the desired L-amino acid are additionally reinforced or in which the metabolism pathways reducing the formation of the desired L-amino acid are eliminated at least in part.