Adenylyl Cyclase, Gene Sequence Encoding Adenylyl Cyclase, Vectors and Cells, and Uses Thereof

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a novel form of adenylate cyclase, an enzyme responsible for synthesizing the important cellular messenger cAMP, along with the gene sequences, vectors, and cells for its expression. The engineered cyclase is structurally modified to enhance its activity—about 4 to 5 times higher than the wild type—by removing certain membrane-spanning segments, making cAMP production independent of external signaling. The invention also includes DNA sequences and cell lines expressing these cyclases, providing efficient tools for research, biotechnology, and screening pharmaceuticals.

Use CasesContent extracted from patent full text and abstract with AI.

• Cell-based screening assays for drug discovery, especially for drugs targeting G-protein coupled receptors (GPCRs)
• Production of engineered cell lines with high cAMP levels for research or biosensor development
• Biosensors to optically detect cAMP changes in living cells via ion channel and fluorescence readouts
• In vitro production of chemicals or fine chemicals by increasing gene expression through cAMP pathways
• Modifying cellular functions and metabolic pathways in biotechnology contexts
• Testing the function or efficacy of potential agonists/antagonists for GPCRs or other signaling pathways
• Accelerating and automating high-throughput pharmacological screening processes
• Enhancing production of amino acids or other biochemical products in industrial microbial or mammalian cell cultures
• Studying cAMP-dependent signaling and gene regulation in basic science

BenefitsContent extracted from patent full text and abstract with AI.

• Significantly increases basal cAMP production in cells, eliminating the need for prior artificial stimulation
• Enables faster, cost-effective, and automated screening assays for pharmaceuticals and signal transduction studies
• Improves sensitivity and throughput of biosensor systems for monitoring intracellular cAMP
• Facilitates efficient studying of GPCRs and their modulators, critical targets for many drugs
• Boosts gene expression and biochemical production in engineered cells for industrial or research use
• Reduces time and complexity of cAMP-dependent assay procedures, potentially halving experimental times
• Allows broader use across many species and cell types due to design versatility
• Provides robust tools (genes, vectors, cell lines) for both academic research and commercial R&D in biochemistry and pharmacology

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Inventors & Applicants

Patent Abstract

The invention relates to an adenylate cyclase, a gene sequence coding for adenylate cyclase, vectors and cells, as well as the use thereof. According to the invention, a membranous adenylate cyclase is used, comprising only one hydrophobic sequence segment which traverses the cell membrane, for example, six times. The first hydrophobic sequence segment and, optionally, the naturally occurring N terminus of the adenylate cyclase is deleted. In this way, the C1 domain projects freely into the interior of the cell containing the adenylate cyclase. The activity of the inventive adenylate cyclase is increased by a factor of 4 to 5 compared to the adenylate cyclase that occurs naturally in the cell.