Method for Producing a Device for Detection of an Analyte, and Device and Use Thereof

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention concerns a method for producing a highly miniaturized, spatially resolved electrochemical biosensor device capable of detecting analytes such as neurotransmitters. The device is fabricated by creating a chessboard-like array of intersecting insulated conductor tracks with individually passivated and isolated nanocavities at their crossing points. Each nanocavity contains a pair of closely spaced electrodes enabling redox-cycling amplification, which significantly increases detection sensitivity and spatial resolution. The particular fabrication process ensures these nanocavities are well isolated from each other, thus preventing signal crosstalk and enabling dense sensor integration.

Use CasesContent extracted from patent full text and abstract with AI.

• Real-time and high-resolution detection of neurotransmitters (e.g., dopamine, serotonin) released by neurons in neurobiology research.
• Development of Lab-on-a-Chip devices for clinical diagnostics or environmental monitoring.
• Point-of-care disease diagnostics based on electrochemical detection of biomolecules in blood, saliva, or other fluids.
• Pharmaceutical drug screening platforms measuring compound effects on neurotransmitter release.
• Integration into portable or wearable biosensors for continuous health monitoring.

BenefitsContent extracted from patent full text and abstract with AI.

• Greatly improved sensitivity due to redox-cycling signal amplification, allowing detection of very low analyte concentrations.
• High spatial resolution through the dense, isolated arrangement of nanocavity sensors, enabling fine mapping of chemical events.
• Ability to miniaturize sensor arrays for high-density integration, crucial for multiplexing and compact devices.
• Biocompatible construction suitable for direct cell culture and in situ measurements with live cell systems.
• Reduced cross-talk between sensors thanks to precise fabrication and isolated nanocavities, ensuring reliable, localized measurements.
• Scalable and efficient fabrication process using lithographic steps, suitable for mass production.

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Patent Abstract

The invention relates to a method for producing a device for detecting an analyte, comprising the following steps: a) a first conductor track having an electrode function is arranged on an insulating substrate, b) a first passivation layer is arranged on the conductive track, c) the passivation layer is opened in a locally limited area so that a locally limited area of the conductive track is exposed, d) a sacrificial layer is arranged in the opening on the conductive track, e) an electrode is arranged on the sacrificial opening, f) a second conductive track is arranged orthogonally to the first conductive track, wherein the second conductive track contacts the electrode, g) a second passivation layer is arranged on the electrode and on the second conductive track, h) the second passivation layer and the electrode are opened, so that the sacrificial layer is exposed, i) the sacrificial layer is removed. The invention relates further to a correspondingly configured device and to the use thereof.