Method for Selectively and Reversibly Adsorbing Nucleic Acids on a Carrier Material

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This invention provides a method for selectively and reversibly binding nucleic acids (such as DNA or RNA) onto a carrier material, such as membranes or filters (including nitrocellulose or hydrophilized nylon), from solutions with high concentrations of kosmotropic salts (like phosphate, sulfate, or citrate). The bound nucleic acids can later be washed and efficiently eluted (released) using aqueous solutions with lower salt concentrations. This process enables the quick and effective isolation, purification, concentration, and desalting of nucleic acids without using labor-intensive techniques like ultrafiltration or gel filtration.

Use CasesContent extracted from patent full text and abstract with AI.

• Laboratory-scale purification of DNA/RNA for molecular biology research.
• Automated high-throughput nucleic acid purification in clinical diagnostics.
• Industrial-scale production or preparation of highly purified plasmid DNA for gene therapy or vaccine manufacturing.
• DNA/RNA sample clean-up prior to PCR, sequencing, or cloning.
• Separation and concentration of target nucleic acids from complex biological mixtures.

BenefitsContent extracted from patent full text and abstract with AI.

• Rapid, efficient, and cost-effective purification and desalting of nucleic acids.
• Reversible and selective binding allows targeted recovery of specific nucleic acids (e.g., DNA over RNA).
• Scalable and suitable for both manual and automated/industrial processes.
• Reduces reliance on time-consuming or less scalable methods like ultrafiltration and gel filtration.
• Minimizes product dilution and loss during purification.
• Enables high purity by removing contaminants such as proteins and RNA from DNA samples.
• Simplified process with easy handling and potential for high-throughput automation.

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Patent Abstract

A method for selective, reversible adsorption of nucleic acids on a substrate, comprises contacting nucleic-acid mixture with the substrate, in which the nucleic acids are adsorbed, purifying nucleic acids with an ethanol/isopropanol or a precipitating agent, and removing the nucleic acids bounded on the substrate by an aqueous solution exhibiting 1 mole of salt content, where the nucleic-acid mixture exhibits 1 mole of kosmotrope salts content, the kosmotrope is a sulfate, citrate or phosphate, and the substrate is a membrane and a filter. The method for selective, reversible adsorption of nucleic acids on a substrate, comprises contacting nucleic-acid mixture with the substrate, in which the nucleic acids are adsorbed, purifying nucleic acids with an ethanol/isopropanol or a precipitating agent, and removing the nucleic acids bounded on the substrate by an aqueous solution exhibiting 1 mole of salt content. The nucleic-acid mixture exhibits 1 mole of kosmotrope salts content. The kosmotrope is a sulfate, citrate or phosphate. The substrate is a membrane and a filter. The filter is a nitrocellulose filter or a hydrophilized nylon filter. The adsorbent consists of silica surfaces. In the contacting process, more than 1 molar solution of a kosmotrope salt is used. The purification is carried out after the aqueous two-phase separation of nucleic acids.