Method for the Production of Recombinant Proteins at Constant pCO2 Content in the Medium

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a method for producing recombinant proteins in eukaryotic cells (especially animal cells like CHO) that are genetically modified in the citrate cycle. The key innovation is maintaining the level of dissolved carbon dioxide (pCO2) in the cell culture medium at a constant and elevated range (10% to 20%), which is achieved using a sophisticated control system. This controlled environment significantly improves protein yield, cell viability, metabolic stability, and allows for extended culture durations at high cell densities.

Use CasesContent extracted from patent full text and abstract with AI.

• Industrial-scale production of therapeutic proteins (such as antibodies, growth factors, interferons, and fusion proteins) for pharmaceuticals.
• Production of recombinant proteins for research, diagnostics, or vaccine manufacturing.
• Bioprocess optimization in mammalian cell culture systems, particularly where metabolic engineering is used to enhance productivity.
• Development of advanced bioreactor control systems for precise gas and metabolic parameter maintenance.
• Enabling robust, high-density fed-batch or continuous cell culture processes in biotechnology and biomanufacturing.

BenefitsContent extracted from patent full text and abstract with AI.

• Significantly higher yields and product concentrations, reducing the costs and scale of downstream protein purification.
• Enhanced process robustness with improved cell viability and longer productive culture phases.
• Ability to manipulate intracellular pH and metabolism for optimized protein expression and quality.
• Flexible application to a range of eukaryotic host cells, especially mammalian and insect systems.
• Potential to double product titers or extend production time, thereby increasing production efficiency and economic output.
• Compatibility with advanced and scalable bioreactor systems, making the technology suitable for large-scale commercial manufacturing.

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Patent Abstract

A method for the recombinant production of a polypeptide in a eukaryotic host cell modified in the citrate cycle is described, wherein the method comprises cultivating the eukaryotic host cell in a suitable medium under conditions which allow the expression of the polypeptide, wherein the content of dissolved CO2 (pCO2) in the medium is maintained at a constant value in the range of 10% to 20%.