Method for Incorporation of Lysosomal Transmembrane Proteins into Cellular Membranes

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This invention describes a method for integrating lysosomal transmembrane proteins into cellular membranes by using a specialized fusion process involving lysosomes and engineered liposomes. The process maintains the structural and functional integrity of the proteins during transfer and fusion with target cellular membranes.

Use CasesContent extracted from patent full text and abstract with AI.

• Treatment of lysosomal storage disorders by replacing defective membrane proteins in patient cells
• Delivery of functional transmembrane proteins into cells for research or therapeutic purposes
• Development of advanced gene and cell therapies targeting membrane protein deficiencies
• Production of engineered cells with customized membrane properties for drug screening or biotechnology
• Modeling of lysosomal diseases in vitro for drug development and research

BenefitsContent extracted from patent full text and abstract with AI.

• Allows precise and functional integration of lysosomal membrane proteins into target cell membranes
• Maintains protein structure and function during transfer, increasing therapeutic potential
• Facilitates development of targeted treatments for diseases caused by transmembrane protein deficiencies
• Enables advanced cell engineering techniques for research and biotechnology applications
• Can potentially reduce side effects compared to traditional gene or protein therapies by enabling more targeted delivery

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Patent Abstract

The invention relates to a method for incorporating lysosomal transmembrane proteins into cellular membranes comprising the steps: a) separation of intact lysosomes from the cell, wherein the transmembrane proteins of the lysosomal membranes remain structurally and functionally intact, b) preparation of fusion liposomes with molecular species A, B, and C, wherein for molecular species A a cationic, amphipathic molecule, for molecular species B an aromatic molecule and for molecular species C a neutral helper lipid are selected, c) preparation of fusogenic lyso-liposomes, wherein the lysosomes fuse with the fusion liposomes, and d) fusion of the fusogenic lyso-liposomes with a cell membrane, wherein the order between step a. and b. can be reversed and in which for the preparation of the fusion liposomes in step b. three molecular species A:B:C of DOTAP:DiO:DOPE are selected in the ratio 1:0.1:1 mol/mol.