Method for Producing a Fusion Mixture for Transferring a Charged Molecule into And/or Through a Lipid Membrane

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent presents a method for producing a specialized fusion mixture that efficiently delivers charged molecules (such as DNA, RNA, proteins, or drugs) into or through lipid membranes, like those found in living cells. The process involves creating fusogenic liposomes from a mixture of specific lipid components, neutralizing the charge on the target molecule, and incubating it with the liposomes to achieve direct fusion and transfer, thereby avoiding common drawbacks of existing delivery methods (like toxicity or poor efficiency).

Use CasesContent extracted from patent full text and abstract with AI.

• Gene therapy: Delivery of genetic material such as DNA or mRNA into cells for therapeutic purposes.
• Cell-based research: Transfection of cells with nucleic acids or other charged molecules for laboratory studies.
• Targeted drug delivery: Transport of charged drugs or therapeutic agents directly into cells or specific organelles.
• Biomedical imaging: Introduction of imaging agents or markers into cell membranes.
• Functionalization of artificial lipid membranes or biosensors.
• Protein or peptide delivery into cells for research or therapeutic applications.

BenefitsContent extracted from patent full text and abstract with AI.

• Higher delivery efficiency of charged molecules compared to traditional methods (lipofection, electroporation).
• Reduced toxicity and lower cellular stress, preserving cell viability and function.
• Minimized degradation of delivered molecules and enhanced retention of their biological activity.
• Applicable to a wide variety of molecular cargos (DNA, RNA, proteins, drugs, nanoparticles, etc.).
• Versatile across different cell types, including primary and difficult-to-transfect cells.
• Details process parameters (pH, ionic strength, sonication) to optimize for various applications.
• Improved reproducibility and control over liposome composition and fusion process.
• Potential for scalable, standardized production of fusion mixtures for clinical or industrial use.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

A method for producing a fusion mixture for a transfer of a charged molecule into and/or through a lipid membrane is disclosed. In an embodiment, the method comprises: providing an initial mixture comprising a positively charged amphipathic molecule A, an aromatic molecule B with hydrophobic range and a neutral, amphipathic molecule C, whereby the molecule types are at hand in a ratio A:B:C of 1-2:0.02-1:0-1 mol/mol; generating a fusogenic liposome by absorption of the initial mixture in a watery solvent; providing a charged molecule; forming a complex from the charged molecule and a neutralizing agent; and incubating the complex with the fusogenic liposome so that a fusion mixture is obtained.