Method of loading a crystallization device

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes an improved method for loading a crystallization device, especially for use in growing membrane protein crystals. The invention involves bringing a matrix-forming compound (usually solid at room temperature) into a fluidic state (e.g., by heating), accurately dispensing it into the device's wells, and then allowing it to solidify. Such pre-filled devices can be stored for a long time and used as ready-to-use consumables for high-throughput and automated crystallization experiments of biopolymers, especially challenging membrane proteins.

Use CasesContent extracted from patent full text and abstract with AI.

• High-throughput screening of crystallization conditions for membrane proteins in pharmaceutical R&D.
• Automated robotic platforms for protein crystallization in structural biology labs.
• Production of ready-to-use crystallization plates for protein X-ray crystallography facilities.
• Academic research into membrane protein structure and function.
• Customization of crystallization devices for varying experimental conditions and additives.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables accurate and reproducible loading of crystallization devices with matrix-forming compounds.
• Facilitates automation and high-throughput approaches in protein crystallization experiments.
• Reduces manual labor, errors, and waste of valuable protein samples.
• Allows long-term storage of pre-filled devices, improving convenience and workflow flexibility.
• Provides flexibility for parallel testing of multiple matrix conditions and additives, optimizing crystallization outcomes.
• Improves reproducibility and standardization in crystallization experiments.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The present invention pertains to a method for loading a crystallization device and for manufacturing a crystallization device comprising multiple receptacles with a pre-defined amount of at least one matrix-forming compound capable of forming a crystallization matrix for a membrane protein, said method comprising the following steps: a) Modifying the state of aggregation of said at least one matrix-forming compound to a fluidic state which allows dispensing said at least one matrix-forming compound, and b) dispensing a defined amount of said at least one matrix-forming compound into at least one receptacle of the crystallization device, wherein said dispensed matrix-forming compound solidifies within said receptacle. Thereby, prefilled crystallization devices are obtained which can be used as consumables in particular in automated crystallization processes. Also provided are protein crystallization methods using respectively prepared crystallization devices.