Methods of Producing 5-ketofructose

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent discloses methods for producing 5-ketofructose, a low-calorie sweetener, by using genetically engineered acetic acid bacteria (preferably Gluconobacter oxydans) that express fructose dehydrogenase. The invention allows conversion of various carbohydrates such as fructose, glucose, sucrose, and starch into 5-ketofructose with high yield and efficiency by employing combinations of specific enzymes through genetic modification. The resulting 5-ketofructose can be isolated and used as a sweetener or as an intermediate for pharmaceutical synthesis.

Use CasesContent extracted from patent full text and abstract with AI.

• Production of 5-ketofructose as a low-calorie, natural-tasting sugar substitute in food and beverages.
• Manufacture of sweetener compositions (powders, solutions, tablets, etc.) for the food industry.
• Development of diabetic-friendly or reduced-calorie food and beverage products.
• Use of 5-ketofructose as a starting material for pharmaceuticals (e.g., synthesis of glycohydrolase inhibitors for medical applications).
• Industrial-scale fermentation and bioprocessing applications for efficient and scalable sweetener production.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables efficient, high-yield production of 5-ketofructose from inexpensive carbohydrates by fermentation, reducing costs.
• Produces a sweetener that is not metabolized by intestinal bacteria, helping support gut health and reducing caloric intake.
• Provides a sugar alternative with taste intensity similar to fructose but with lower caloric content, suitable for diabetics and calorie-conscious consumers.
• Flexible substrate use (fructose, glucose, sucrose, starch) increases process versatility and commercial appeal.
• Genetic modifications improve specificity, minimize byproducts, and maximize conversion efficiency, allowing for scalable and sustainable industrial production.
• Facilitates the preparation of sweetener formulations in various forms for diverse food and beverage applications.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The present invention relates to a method of producing 5-ketofructose using an acetic acid bacterium which is genetically modified to express a fructose dehydrogenase as well as to microorganisms which can be used in said method.