D-xylose Dehydrogenase from Coryneform Bacteria and Process for Preparing D-xylonate

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent introduces a novel D-xylose dehydrogenase enzyme derived from coryneform bacteria, along with the genetic sequences encoding it, and genetically defined bacteria capable of overexpressing this enzyme. The invention also provides processes for efficiently producing D-xylonate from D-xylose using these modified coryneform bacteria strains, offering a non-GMO route by making precise mutations in regulatory regions instead of introducing foreign DNA. This system enables robust, scalable, and industry-friendly microbial production of D-xylonate from renewable resources.

Use CasesContent extracted from patent full text and abstract with AI.

• Industrial-scale production of D-xylonate as a platform chemical from biomass such as plant-derived lignocellulose, cellulose, hemicellulose, or sugarcane bagasse.
• Replacement of D-gluconate with D-xylonate derived from sustainable sources in food, pharmaceutical, and chemical manufacturing.
• Bioprocess development in the food and pharmaceutical industries using non-genetically modified organisms (non-GMO) for easier regulatory approval.
• Utilization of D-xylonate in the formulation of construction materials such as cement and concrete as a water reducer, dispersing agent, or setting retardant.
• Development of safe microbial strains for fermentation-based production of D-xylonate derivatives for use in polymers, solvents, dyes, and animal feed additives.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables high-yield, cost-effective microbial production of D-xylonate on defined media with robust, well-understood coryneform bacteria.
• Non-GMO approach increases acceptability in food and pharmaceutical sectors by avoiding foreign gene insertion and associated regulatory complexity.
• Facilitates the use of renewable second-generation feedstocks (e.g. bagasse, lignocellulose) to produce valuable platform chemicals, supporting a bio-based economy.
• Offers flexibility for genetic optimization without detrimental effects on bacterial physiology, as changes are limited to specific regulatory regions.
• Provides a more stable production platform compared to previous systems that used foreign genes or required complex nutrient media, reducing cost and operational risk.
• Broadens potential industrial, food, and biomedical applications for D-xylonate and its derivatives, driven by scalable, safe, and sustainable production methods.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The invention relates to D-xylose-dehydrogenase from coryneform bacteria, nucleic acid sequences encoding same, corresponding living organisms, preferably coryneform bacteria, which contain a D-xylose-dehydrogenase with increased expression and/or D-xylose-dehydrogenase activity, and corresponding uses. The present invention also relates to a process for preparing D-xylonate including D-xylose-dehydrogenase from coryneform bacteria.