Method for Quantifying Protein Aggregates of a Protein Misfolding Disease in a Sample

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention provides a highly sensitive method and device for detecting and quantifying protein aggregates associated with protein misfolding diseases, such as Alzheimer's, in complex biological samples. The method uses specialized substrates with capture molecules (often antibodies) that selectively bind to disease-related protein aggregates. It includes calibration standards using nanoparticles to enable accurate quantitation and utilizes advanced fluorescence microscopy for detection, allowing precise measurement of even minute concentrations of pathogenic aggregates.

Use CasesContent extracted from patent full text and abstract with AI.

• Early diagnosis and monitoring of neurodegenerative diseases like Alzheimer's and Parkinson's by detecting disease-specific protein aggregates in patient samples (e.g., brain tissue, cerebrospinal fluid, blood).
• Screening and evaluation of new therapeutic drugs targeting reduction or elimination of toxic protein aggregates in preclinical and clinical settings.
• Research applications to study aggregation mechanisms and progression of protein misfolding diseases.
• Quality control and validation of biopharmaceutical products where aggregate quantification is critical.
• Detection of other protein misfolding-related diseases in both human and veterinary medicine.

BenefitsContent extracted from patent full text and abstract with AI.

• Ultra-high sensitivity, capable of detecting aggregates down to femtomolar concentrations, far surpassing traditional ELISA methods.
• Specificity for aggregates over monomeric proteins, minimizing background and false positives.
• Applicable to complex biological samples without interference from myriad other proteins present.
• Quantitative results enable precise measurement of disease biomarkers and drug efficacy.
• Flexible use in various sample types (tissues, fluids, cells) and adaptable to different diseases characterized by protein aggregation.
• Facilitates rapid, reliable screening of potential therapeutics and monitoring of treatment effects.

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Patent Abstract

The invention relates to a method for quantifying protein aggregates of a protein misfolding disease in a complex sample. The invention further relates to a device, to a kit and to a use of the device and of the kit.