Process for isolating nucleic acids from a nucleic acid-containing sample and a testkit

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent discloses a process and a test kit for isolating nucleic acids (such as RNA and DNA) from biological samples, especially those with high levels of interfering substances like polysaccharides and secondary metabolites (e.g., plant tissues). The process involves mechanical sample breakdown, use of specific chaotropic denaturants (e.g., guanidine salts), ethanol precipitation, binding of nucleic acids to a mineral carrier, sequential washing (including with a high-salt sodium citrate/NaCl solution), and elution. The accompanying kit contains all required reagents and components to perform this process easily and with improved yield and purity compared to existing methods.

Use CasesContent extracted from patent full text and abstract with AI.

• Extraction of RNA and DNA from plant tissues with high metabolite content for genomics and transcriptomics research.
• Isolation of nucleic acids from genetically modified organisms (plants or animals) for analysis and quality control.
• Sample preparation for PCR, RT-PCR, or sequencing applications in molecular diagnostics.
• Isolation of viral or microbial nucleic acids from environmental or clinical samples for pathogen detection.
• Purification of nucleic acids in pharmaceutical plant research or breeding programs.

BenefitsContent extracted from patent full text and abstract with AI.

• Significantly improved yield and purity of nucleic acids from samples that are otherwise difficult to process, such as those rich in compounds like polysaccharides.
• Reduced use of toxic chemicals compared to traditional methods (e.g., phenol, chloroform), enhancing safety and environmental friendliness.
• Faster and less labor-intensive nucleic acid extraction workflow.
• Versatile — applicable to a wide range of organisms (plants, animals, microbes) and sample types.
• Facilitates downstream molecular biology applications by providing higher quality nucleic acids suitable for sensitive analyses.

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Patent Abstract

Isolating nucleic acids from nucleic acid-containing samples, where the samples are mechanically broken down with known methods, comprises adding a chaotropic denaturing agent to the sample, homogenizing the lysed sample and removing the cell debris by centrifugation, adding alcohol to the sample, separating the nucleic acids by binding the nucleic acids to a mineral carrier, washing the nucleic acids, adding sodium citrate or sodium chloride solution to the nucleic acids, and washing and eluting the adsorbed total nucleic acids according to known methods. An independent claim is included for a test kit for isolating nucleic acids from nucleic acid-containing samples, comprising a buffer containing guanidine hydrochloride, guanidine isothiocyanate or guanidine thiocyanate, ethanol, separation column for nucleic acids, washing buffer, sodium citrate or sodium chloride solution and collecting container.