Process for the Production of D-Xylonate and Coryneforme Bacterium

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This invention presents a process for producing enantiomerically pure D-xylonate using coryneform bacteria, in which the activity of the IolR gene (responsible for regulating myo-inositol metabolism) has been reduced, eliminated, or deleted. The use of these genetically optimized (but non-recombinant) bacteria, such as Corynebacterium glutamicum, enables efficient transformation of D-xylose to D-xylonate with high yields and purity, addressing current industrial challenges related to productivity, cost, and regulatory acceptance in food and pharma sectors.

Use CasesContent extracted from patent full text and abstract with AI.

• Industrial production of D-xylonate for use as a precursor in the manufacture of polyamides, polyesters, and 1,2,4-butanetriol.
• Sustainable biosynthesis of platform chemicals from lignocellulosic biomass for the chemical industry.
• Production of D-xylonate for application in pharmaceuticals, such as in drug synthesis pathways that require enantiomerically pure acids.
• Manufacture of food additives or ingredients using non-recombinant microorganisms accepted in food processing.
• Development of green biotechnological processes for bulk chemical production, reducing reliance on fossil-based routes.

BenefitsContent extracted from patent full text and abstract with AI.

• High yield and enantiomeric purity (100%) of D-xylonate, increasing process efficiency.
• Use of non-recombinant, GRAS-status organisms (e.g., Corynebacterium glutamicum), simplifying regulatory approval for applications in food and pharma.
• Production can be performed on defined, low-cost media, reducing overall fermentation costs.
• The process avoids genetically modified organisms in the traditional recombinant sense, making it more widely acceptable in sensitive markets.
• Scalable and compatible with standard fermentation technologies, facilitating industrial scale-up.
• Improved downstream processing due to use of defined media and high product specificity.

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Patent Abstract

The invention relates to a method of producing D-xylonate and to a coryneform bacterium. It has been found in accordance with the invention that it is possible to achieve a very high yield of enantiomerically pure D-xylonate, proceeding from D-xylonate as substrate, with coryneform bacteria in which the activity of the IolR gene which encodes a regulator of myo-inositol metabolism, called IolR gene hereinafter, has been reduced or eliminated, or in which the IolR gene has been at least partly deleted. The invention therefore provides a method of preparing D-xylonate and a coryneform bacterium as production strain, in which the activity of the IolR gene in the production strain is reduced or eliminated compared to the wild form or a mutant of the wild form, or wherein the IolR gene has been partly or completely deleted.