Provision of Malonyl-Coa in Coryneform Bacteria and Method for Producing Polyphenoles and Polyketides with Coryneform Bacteria

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention concerns genetically engineered coryneform bacteria (such as Corynebacterium glutamicum) that are specifically modified to increase their internal production of malonyl-CoA, an essential metabolic intermediate for the biosynthesis of valuable polyphenols (such as resveratrol and naringenin) and polyketides. The approach involves precise genetic changes reducing fatty acid synthase activity, altering specific enzymes' regulatory regions, and/or deactivating metabolite degradation pathways, enabling efficient, scalable microbial production of polyphenols and polyketides using safe (GRAS-status) bacterial factory strains without reliance on externally added inhibitors or recombinant DNA.

Use CasesContent extracted from patent full text and abstract with AI.

• Industrial microbial fermentation for the production of health-related polyphenols like resveratrol (anti-aging, anti-inflammatory, anti-tumor applications).
• Production of polyketides for pharmaceuticals, nutraceuticals, or food additives.
• Sustainable, scalable synthesis of plant secondary metabolites for drug development and research.
• Biosynthesis of flavor or antioxidant compounds for food and beverage industries.
• Biotechnological production of feed supplements with health-promoting properties.
• Platform for engineering further valuable secondary metabolites by combining with other biosynthetic genes.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables high-yield, cost-effective, and scalable microbial production of polyphenols and polyketides, overcoming limitations of plant extraction and chemical synthesis.
• Uses coryneform bacteria, which are GRAS-certified and widely accepted in food and pharma, facilitating regulatory approval.
• Avoids growth-inhibiting and costly chemical inhibitors like cerulenin, improving cell health and process economics.
• Allows non-GMO (non-recombinant) modification routes, which can simplify regulatory approval and expand potential uses, especially for food and feed.
• Minimizes by-product formation and product degradation by deleting relevant metabolic pathways within the bacteria, boosting yield and purity.
• Supports flexible genetic engineering, supporting a broad array of biosynthetic targets for future products.

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Patent Abstract

The invention relates to a coryneform bacteria cell having an increased provision of malonyl CoA with respect to its type, a protein for a fatty acid synthase FasB with reduced functionality and nucleic acid sequence coding therefor. The invention also relates to a method for the increased provision of Malonyl-CoA and to a method for producing polyketides or polyohenoles with coryneforme bacteria cells and to the uses thereof.