Fusion Mixture for Lipid-Containing Membrane Modification of an Arbitrary Lipid Membrane, a Cell Membrane, a Component of a Cell Membrane, or a Cell Membrane Separated from Other Cellular Components in Vivo or in Vitro

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent discloses a novel fusion mixture designed for the modification of lipid-containing membranes (such as cellular or synthetic membranes), using just two essential types of molecules: a positively charged amphipathic molecule (type A) and an aromatic molecule (type B), without the need for the neutral 'helper lipids' traditionally used. The mixture can be tailored within a broad range of ratios (A:B of 1:0.02 to 1:2 mol/mol), allowing simple and efficient membrane fusion in vitro or in vivo for various applications, including delivery of diverse functional substances into target membranes or cells.

Use CasesContent extracted from patent full text and abstract with AI.

• Targeted delivery of drugs (e.g., chemotherapeutics) directly into cells by fusion of drug-loaded liposomes with cell membranes.
• Rapid and efficient transfection of cells with nucleic acids, such as plasmid DNA or RNA.
• Selective labeling and fluorescence imaging of cell membranes for research or diagnostic applications.
• Membrane biotinylation for cell separation or purification using magnetic beads.
• Incorporation of membrane proteins or other biological molecules into synthetic or cellular membranes, for research or therapeutic purposes.
• Use in developing synthetic biology tools, including artificial cells or organelles with customized membranes.
• Delivery of nanoparticles (magnetic, fluorescent, etc.) into cells for biomedical research or diagnostics.

BenefitsContent extracted from patent full text and abstract with AI.

• Simplifies the composition of fusion mixtures by eliminating the need for neutral helper lipids, reducing the system to only two core molecules.
• Offers a broad working concentration range, making the system flexible and adaptable to different membrane types and applications.
• Enables rapid membrane fusion (in minutes) and efficient delivery of functional molecules into membranes or cells.
• Is highly versatile, allowing incorporation of various cargoes (nucleic acids, proteins, drugs, nanoparticles, etc.) for multiple purposes.
• Shows high biocompatibility with cells, preserving cell viability during fusion.
• Improves delivery efficiency compared to traditional endocytosis-based methods, with more homogeneous results (e.g., in transfection).
• Supports sequential or repeated fusion steps for complex membrane modification workflows.
• Facilitates scalable and straightforward preparation using conventional laboratory solvents and protocols.

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Inventors & Applicants

Patent Abstract

The invention relates to a fusion mixture for lipid-containing membrane modification of any desired target membrane, a cell membrane, a constituent part of a cell membrane or a cell membrane separated from the remaining cell constituent parts in vivo or in vitro, comprising a positively charged amphipathic molecule A and an aromatic molecule B, wherein the molecule type A and the molecule type B are present in a ratio A:B of 1:0.02 to 1:2 mol/mol.