Method for Detection of Extracellular Vesicles in a Sample

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention discloses a method and kit for detecting extracellular vesicles (EVs) in a sample. The process involves applying the sample to a substrate, adding probe molecules that selectively bind to the EVs and emit a measurable signal, and then detecting the signal to confirm the presence and possibly the concentration and type of EVs. This approach reduces the need for complex sample preparation, allows for both qualitative and quantitative analysis, and can be used with various types of samples, such as body fluids, cell cultures, or environmental materials.

Use CasesContent extracted from patent full text and abstract with AI.

• Non-invasive medical diagnostics by detecting EVs in blood, urine, or cerebrospinal fluid samples to monitor diseases such as cancer, cardiovascular, or kidney disorders.
• Therapy monitoring and assessment of drug efficacy by quantifying and characterizing EVs before and after treatment.
• Pharmaceutical research for characterizing and profiling EVs during drug development.
• Basic research into cell communication, biomarker discovery, and understanding the role of EVs in various biological processes.
• Screening and stratification of patients for clinical studies based on levels or types of EVs.
• Quality control and standardization in the manufacture of biologics involving EVs.
• Environmental monitoring by detecting EVs in water, soil, or plant samples.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables sensitive and specific detection of extracellular vesicles without extensive sample purification.
• Allows for both quantitative (number/concentration) and qualitative (type/characteristics) analysis of EVs.
• Can be applied to a wide range of sample types, including minimally processed clinical or environmental samples.
• Reduces analysis time and complexity compared to conventional techniques like electron microscopy or flow cytometry.
• Kit format makes the technology accessible and easier to standardize across laboratories.
• Potentially supports multiplexed or high-throughput analyses for screening and clinical diagnostics.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The invention relates to a method for detecting extracellular vesicles in a sample, said method involving the following steps: a) applying the sample to a substrate; b) adding probes which are suitable for the detection and which mark the extracellular vesicles by specifically binding thereto; and c) detecting the extracellular vesicles by measuring a specific probe signal. Step b) can be carried out prior to step a). A kit for carrying out said method is also disclosed.