Production of Viruses in Continuously Growing Epithelial Cell Lines Derived from Chicken Gut

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes the use of specific, continuously growing avian epithelial cell lines derived from chicken gut for efficient in vitro production of viruses. These cell lines enable significantly higher virus yields compared to traditional cell lines (such as MDCK cells) or embryonated chicken eggs, streamlining the production of viral vaccines without relying on eggs.

Use CasesContent extracted from patent full text and abstract with AI.

• Production of human vaccines (e.g., for influenza) using cell-culture-derived viruses
• Manufacture of veterinary vaccines for poultry diseases (e.g., Newcastle disease)
• Rapid mass production of pandemic and seasonal influenza vaccines
• Development of multivalent vaccines combining multiple virus strains
• Production of viruses for research or diagnostic purposes without using animal-derived eggs

BenefitsContent extracted from patent full text and abstract with AI.

• Achieves up to 10 times higher virus yields compared to established cell lines, increasing production efficiency
• Enables faster response and manufacturing of vaccines during pandemics or outbreaks
• Circumvents reliance on embryonated chicken eggs, overcoming supply and allergen issues
• Suitable for producing a broad range of viruses, including those infecting humans and birds, supporting diverse vaccine needs
• Cell lines are immortalized and easy to passage, offering consistent and scalable virus production
• Reduces the risk of contamination or variability associated with egg-based vaccine production

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Inventors & Applicants

Patent Abstract

The invention relates to the use of cells of an in vitro cell culture for the production of viruses. Thereby, the cells are avian epithelial cells chosen from at least one of the cell cultures deposited under accession numbers DSM ACC3345, DSM ACC3346, DSM ACC3347, DSM ACC3348, DSM ACC3349 at Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH on December 12, 2018. The invention further relates to related manufacturing methods.