Method for studying virus particle release

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention relates to genetic constructs and methods for studying the release of influenza virus particles, focusing on how the matrix protein M1 associates with the cellular membrane. It introduces specially engineered M1 mutants that are fused with a fluorescent protein (such as CFP), and further equipped with either a nuclear export signal or a myristoylation signal, which helps target the fluorescent M1 to the correct cellular compartment. These tools allow researchers to visually monitor and analyze the binding of viral proteins to cell membranes using fluorescence microscopy, and to screen for compounds that can interfere with this process, which is crucial for virus assembly and release.

Use CasesContent extracted from patent full text and abstract with AI.

• Screening for new antiviral drugs that block influenza virus particle assembly or release by monitoring the effect of candidate compounds on M1-membrane association.
• Basic research into the molecular mechanisms of influenza virus assembly, especially the role of matrix protein M1 in viral budding.
• Developing high-throughput assays for pharmaceutical or academic research to quickly test many compounds for anti-influenza activity.
• Studying protein-lipid interactions in the context of viral replication and cell biology.
• Education and training purposes to teach fluorescence-based protein tracking or virus-cell interaction methods.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables direct visualization and quantification of viral protein-membrane interactions in living cells in real-time.
• Allows for high-throughput screening of potential antiviral compounds, accelerating drug discovery.
• Avoids limitations of conventional biochemical methods, providing more specific and reliable analysis of protein localization and interaction.
• Facilitates a better understanding of virus assembly pathways, which may be useful for future vaccine or therapy development.
• Flexible and adaptable system: compatible with different fluorescent proteins or signals, suitable for various influenza strains or research needs.

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Inventors & Applicants

Patent Abstract

The present invention refers to methods and means for studying influenza virus particle release, in particular matrix protein M1 association with the cellular membrane. For this purpose, a matrix protein M1 mutant comprising a nuclear export signal of Rev protein or a myristoylation signal of Lyn kinase, and further comprising a fluorescent protein is provided, as well as nucleic acids related thereto.