A method for the quantitative analysis of RNA molecules in a sample

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention describes a method to quantitatively measure the amount of RNA molecules in a sample. The process involves converting RNA into complementary DNA (cDNA) using specially designed oligonucleotides, amplifying the cDNA through enzymatic reactions, and then analyzing the resulting DNA to determine the amount of the original RNA.

Use CasesContent extracted from patent full text and abstract with AI.

• Medical diagnostics for detecting RNA viruses (e.g., COVID-19, HIV)
• Gene expression research in biological and clinical laboratories
• Monitoring patient responses to treatments at the molecular level
• Biotechnology research for developing new drugs or therapies
• Agricultural analysis for detecting plant pathogens or gene expression in crops

BenefitsContent extracted from patent full text and abstract with AI.

• Highly sensitive and specific detection of RNA molecules
• Quantitative results allow for precise measurement of RNA levels
• Applicable to a wide range of biological and clinical samples
• Facilitates rapid and accurate molecular diagnostics
• Enables researchers and clinicians to monitor genetic changes over time

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Patent Abstract

The invention relates to a method for the quantitative analysis of RNA molecules comprising the following steps: a) performing a reverse transcription reaction with a first oligonucleotide molecule to yield cDNA molecules, wherein the first oligonucleotide molecule comprises - a first sequence portion that hybridizes with the RNA molecules at, - a second sequence portion that provides for a sequence for binding of a third oligonucleotide to prime an enzymatic DNA amplification reaction, b) performing an enzymatic DNA amplification reaction to amplify the cDNA molecules, comprising: - a second oligonucleotide molecule, comprising a first sequence portion for hybridizing with the cDNA molecules, and a second sequence portion that provides for a sequence for binding of a fourth oligonucleotide to prime the enzymatic DNA amplification reaction, - a third oligonucleotide molecule for priming an enzymatic DNA amplification reaction, and - a fourth oligonucleotide molecule for priming an enzymatic DNA amplification reaction, and c) performing a quantitative analysis of the amplified DNA molecules.