Method for Producing a Polyglycerin Nanogel for Encapsulation and Release of Biologically Active Substances

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This patent describes a gentle, surfactant-free method for producing polyglycerin nanogels that can encapsulate and release biologically active substances, such as proteins, enzymes, peptides, nucleic acids, and hormones. The process leverages bioorthogonal click chemistry reactions between modified polyglycerin macromonomers in water-miscible organic solvents, enabling the formation of highly stable, pH-responsive nanogels without damaging sensitive biological compounds. The nanogels can then protect the encapsulated substances during delivery and release them in a controlled manner, especially under acidic conditions (such as those found in disease sites).

Use CasesContent extracted from patent full text and abstract with AI.

• Controlled drug delivery systems for sensitive protein or enzyme therapeutics, allowing on-site release (e.g., in cancer or inflamed tissue).
• Encapsulation and stabilization of labile biomolecules (such as hormones, antibodies, or nucleic acids) for pharmaceutical applications.
• Targeted delivery platforms for gene therapy, peptide-based drugs, or biologicals in human medicine.
• Development of bioresponsive or stimuli-responsive nanocarriers that release drugs in response to pH changes found in specific tissues or disease microenvironments.
• Preservation and controlled release of enzymes or proteins in biotechnology (e.g., industrial or diagnostic enzymes requiring protection from denaturation).

BenefitsContent extracted from patent full text and abstract with AI.

• Allows efficient encapsulation of sensitive, hydrophilic biomolecules without loss of activity due to mild processing conditions (no harsh chemicals, surfactants, or ultrasound).
• Highly biocompatible and non-toxic materials (polyglycerin), enhancing safety for biomedical uses.
• pH-responsiveness enables site-specific and controlled release of therapeutic payloads, improving efficacy and minimizing side effects.
• Elimination of surfactants and copper catalysts reduces risks associated with impurities and toxic residues, particularly important for pharmaceuticals.
• Nanogel particle size can be precisely tuned by adjusting process parameters, enabling customization for different applications.

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Inventors & Applicants

Patent Abstract

The invention relates to a method for producing a polyglycerin nanogel, comprising the following steps: mixing an aqueous solution of first polyglycerin macromonomers (1) which are modified with a first reactive group (10), comprising an aqueous solution of second polyglycerin macromonomers (2) which are modified with a second reactive group (20), wherein the first reactive group (10) and the second reactive group (20) can react with one another, forming a chemical bond; transferring the mixture into an organic non-solvent (4); and precipitating a polyglycerin nanogel (6) which consists of first polyglycerin macromonomers (1) and second polyglycerin macromonomers (2) covalently bonded to one another. The invention is characterised in that the organic non-solvent (4) is miscible with water (3) and that the method is carried out without the addition of surfactant substances.