Means and method for the production of transgenic plants that are resistant to clubroot

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This patent describes a genetic engineering method to produce transgenic plants that are resistant to clubroot disease, which is caused by Plasmodiophora brassicae. The core invention involves inserting a DNA sequence into plants that includes a specific promoter (mostly active in the plant's hypocotyls and roots, but not in the shoot apex) linked to a gene encoding a protein that decreases cytokinin levels. This tissue-specific expression reduces susceptibility to clubroot without negatively affecting the normal growth of the shoot, addressing a key limitation in previous approaches.

Use CasesContent extracted from patent full text and abstract with AI.

• Development of clubroot-resistant crops in the Brassicaceae family, such as canola, cabbage, broccoli, and cauliflower.
• Transformation of other economically important plants prone to clubroot or similar hormone-mediated diseases.
• Breeding of high-yielding, disease-resistant plants where shoot growth and economic performance are crucial.
• Research in plant hormone regulation and disease resistance.
• Production of seeds and propagation material for resistant crop varieties.

BenefitsContent extracted from patent full text and abstract with AI.

• Confers resistance to clubroot disease while maintaining normal shoot growth, improving crop yield and quality.
• Targets cytokinin reduction only in relevant tissues (hypocotyls/roots), minimizing negative plant growth side effects.
• Allows for stable, heritable disease resistance across plant generations.
• Reduces dependency on chemical fungicides or crop rotation to control clubroot, contributing to more sustainable agriculture.
• Can be adapted to various Brassicaceae crops, offering broad agricultural utility.

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Patent Abstract

The present invention refers to an isolated nucleic acid, comprising a) at least one promoter sequence which is predominantly active in the hypocotyls compared to shoot apex tissue of a plant; and b) a nucleic acid sequence encoding for a protein that is able to decrease a cytokinin status in a plant tissue; wherein the nucleic acid sequence encoding said protein is located in 3'-position to the at least one promoter and wherein the at least one promoter sequence and the nucleic acid sequence encoding said protein are functionally linked with one another.