Increase of Protein Expression and Secretion by Artificial Co-Expression of Hdlbp/vigilin

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention provides a method and platform to enhance the production and secretion of recombinant proteins in eukaryotic cells by artificially co-expressing the RNA-binding protein HDLBP (also known as Vigilin) alongside the protein of interest. By increasing the intracellular levels of HDLBP and/or using codon-optimized genes (boosting CU-rich codons), the yield of secreted proteins—such as therapeutic antibodies, enzymes, hormones, and cytokines—can be greatly improved. The strategy is compatible with a range of standard mammalian and insect host cell lines widely used in biotechnology.

Use CasesContent extracted from patent full text and abstract with AI.

• Biopharmaceutical manufacturing – high-yield production of therapeutic proteins (e.g., antibodies, insulin, EPO, cytokines) for clinical use.
• Industrial enzyme production for diagnostics, food processing, or chemical synthesis.
• Research labs requiring efficient secreted protein expression for structural biology, functional assays, or antibody generation.
• Development of commercial cell lines optimized for high protein secretion capabilities.
• Production of fusion proteins or nanobodies for therapeutic or diagnostic purposes.

BenefitsContent extracted from patent full text and abstract with AI.

• Significantly increases the expression and secretion of recombinant proteins (up to three-fold or more).
• Applicable to a wide variety of proteins and host cell lines commonly used in industry (CHO, HEK293, insect cells, etc.).
• Works synergistically with codon optimization strategies for even higher yields.
• Improves cost-effectiveness of protein production by maximizing output per cell batch.
• Facilitates scalable and flexible manufacturing of protein therapeutics and research reagents.
• Potential for augmenting existing production cell lines with minimal changes to established protocols.

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Patent Abstract

The invention is related to a method for producing a secreted recombinant protein of interest in a cell, wherein the method comprises artificially co-expressing a protein different from the secreted recombinant protein of interest.