Screening of Conjugated Antibodies

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This patent describes a method for site-specific functionalization of antibodies (immunoglobulins), especially for creating and screening antibody-drug conjugates (ADCs). Using the enzyme transglutaminase, the method enables precise and uniform attachment of drugs or other functional groups to specific sites (glutamine residues) on the antibody's constant region, without modifying the antigen-binding regions. This allows for effective screening and comparison of different antibodies or antibody-drug combinations for desired properties like cytotoxicity, stability, and specificity.

Use CasesContent extracted from patent full text and abstract with AI.

• Development and screening of antibody-drug conjugates (ADCs) for cancer therapy.
• Generation of diagnostic antibodies with attached labels for imaging or detection.
• Creation of antibodies with improved pharmacokinetics by conjugating relevant moieties.
• Batch production of homogeneous antibody conjugates for clinical or research use.
• Screening candidate antibodies from hybridomas or libraries to identify the best functional conjugate.
• Production of customized antibody therapeutics targeting infections, autoimmune diseases, or inflammation by attaching various drugs or effector molecules.

BenefitsContent extracted from patent full text and abstract with AI.

• Highly site-specific and stoichiometric antibody conjugation, leading to uniform products.
• Preserves antibody binding affinity by not modifying the antigen-binding region.
• Allows direct comparison of different antibodies in their functionalized form, improving screening efficiency.
• Applicable to a wide range of antibodies (rodent, human, chimeric, etc.), including unpurified samples.
• Suitable for conjugating large, hydrophobic, or charged drugs that are challenging for other conjugation methods.
• Enables production of antibody-drug conjugates with narrow drug-to-antibody ratios, increasing reproducibility and therapeutic consistency.
• Potentially reduces cost and time in ADC development by simplifying the screening and conjugation workflow.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The present invention relates to a method for the functionalization of immunoglobulins through the use of transglutaminase, including methods for screening functionalized antibodies for characteristics of interest, antibody compositions comprising a plurality of functionalized antibodies, and functionalized antibodies with rodent constant regions.