Modified Mussel Proteins, Uses Thereof and Related Compounds

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention introduces genetically engineered mussel proteins in which specific amino acid residues (mainly tyrosine) are replaced with novel derivatives of 3,4-dihydroxyphenylalanine (Dopa) that are 'protected' with photocleavable groups, like ortho-nitrobenzyl (ONB). These modifications allow the adhesive properties of the protein to be precisely activated by UV light, mimicking and enhancing natural underwater mussel adhesion. The patent also covers the DNA sequences encoding these modified proteins, the tRNA synthetase and tRNA tools used to incorporate the protected Dopa residues, and methods of producing and activating these bio-adhesives for use.

Use CasesContent extracted from patent full text and abstract with AI.

• Biocompatible, photo-activated surgical glues for bonding tissues or repairing bone fractures during surgery.
• Surface coatings for medical devices or implants to improve adhesion or impart antimicrobial properties.
• Development of biodegradable wound dressings that promote healing and can be activated on demand.
• Precision-controlled adhesive layers for biomedical engineering or material science applications, where adhesion can be triggered by light.
• Research tools for studying adhesion mechanisms or developing new biomaterials with tunable sticking properties.
• Temporary adhesives in manufacturing or assembly processes that require on-demand activation and biocompatibility.

BenefitsContent extracted from patent full text and abstract with AI.

• Allows precise spatial and temporal control of adhesive activation using safe UV light (photoactivation).
• Biodegradable and biocompatible, reducing toxicity risks compared to synthetic glues like cyanoacrylates.
• Strong adhesion even under wet (physiological) conditions, mimicking natural mussel adhesive strength.
• Reduced likelihood of premature adhesive activation (thanks to protected Dopa residues) and less risk of oxidative degradation.
• Potential for easy and scalable production in engineered microbes or cell-free systems.
• Can be functionalized with antimicrobial peptides for infection-preventing medical coatings.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The invention relates to modified mussel proteins, to uses thereof, to nucleotide sequences encoding the modified mussel proteins, to an aminoacyl-tRNA synthetase suited for producing the modified mussel proteins, and to a novel use of an 3,4-dihydroxyphenylalanine derivative comprising a protecting group on at least one hydroxyl residue of its catechol moiety, in particular of ortho-nitrobenzyl-3,4-dihydroxyphenylalanine, that was applied for producing the modified mussel proteins.