3D in vitro bi-phasic cartilage-bone construct

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The invention relates to a three-dimensional (3D) in vitro bi-phasic organoid that mimics the functional interface between cartilage and bone tissues. This organoid consists of an artificial cartilage layer in direct contact with a layer of artificial bone that includes a supporting scaffold and structures similar to bone marrow. The invention provides methods for creating these bi-phasic constructs from mesenchymal progenitor cells and enables their use in research, drug screening, and regenerative medicine, including as transplants or as part of organ-on-a-chip devices.

Use CasesContent extracted from patent full text and abstract with AI.

• In vitro modeling of bone-cartilage interfaces for biological and disease research.
• High-throughput screening of drugs or substances that may affect cartilage or bone tissue.
• Replacement or reduction of animal models in research by providing a more human-relevant tissue model.
• Testing of tissue responses to mechanical forces (e.g., simulating movement or load).
• Regenerative medicine applications, including potential use as transplant material for repairing osteochondral defects or joint damage.
• Integration into organ-on-a-chip systems for studying pharmacodynamics, pharmacokinetics, and tissue interactions in a controlled environment.

BenefitsContent extracted from patent full text and abstract with AI.

• Mimics the natural structure and interaction of cartilage and bone better than previous single-tissue in vitro models.
• Enables study of tissue development, differentiation, and interactions under more physiologically relevant conditions.
• Supports long-term culture, including perfusion for nutrient and oxygen supply, allowing extended experiments.
• Can replace or reduce labor-intensive and costly animal testing with in vitro alternatives.
• Enables personalized medicine opportunities through the use of patient-derived cells, reducing risk of rejection in transplant use.
• Facilitates high-throughput screening of therapeutics and substances for effects on bone or cartilage tissue.
• Organ-on-a-chip integration enables controlled, scalable, and automated testing, including live cell imaging and measurement of tissue responses to both biochemical and physical stimuli.

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Patent Abstract

The present invention is directed to a 3D in vitro bi-phasic cartilage-bone organoid comprising: a) a layer of artificial cartilage tissue; and b) a layer of artificial bone tissue, wherein the artificial bone tissue comprises a structure-giving scaffold and a bone marrow structure; wherein the layer of artificial cartilage tissue contacts at least one surface of the layer of artificial bone tissue, as well as to uses and a method of production thereof.