Method for the Synthetic Cleaning of Peptides without Hplc

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention presents a new method for purifying synthetic peptides that eliminates the need for high-performance liquid chromatography (HPLC). The core innovation is a specially designed linker molecule (A-E-L-R) that can selectively bind, wash, and release peptides from solid phases, using specific chemical reactions. The method is compatible with a wide range of peptide functional groups and can be automated or parallelized for higher efficiency and cost savings. A ready-to-use kit is also described for practical laboratory applications.

Use CasesContent extracted from patent full text and abstract with AI.

• Purification of synthetic peptides for pharmaceutical and biotech research and development.
• High-throughput peptide library screening and synthesis for drug discovery or proteomics.
• Automated peptide manufacturing processes where large numbers of different peptides must be cleaned quickly and efficiently.
• Preparative purification of peptides for therapeutic, diagnostic, or industrial use without HPLC.
• Enabling more accessible peptide synthesis in laboratories lacking chromatographic equipment.

BenefitsContent extracted from patent full text and abstract with AI.

• Removes the need for costly and time-consuming HPLC purification, drastically reducing costs and increasing throughput.
• Compatible with many types of peptides and functional groups, including those problematic for existing catch-and-release or affinity methods.
• Enables automation and parallelization, supporting high-throughput workflows for peptide synthesis and purification.
• The materials (e.g., linker and purification phase) are inexpensive and robust, making scale-up and industrial application easier.
• Reduces solvent and equipment use, thereby lowering environmental impact and operational complexity.
• Can serve as either a stand-alone purification method or as a pre-purification step before finer chromatographic polishing, improving downstream efficiency.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The invention relates to a linker molecule for purifying a peptide according to formula A-E-L-R. The invention also relates to a method for purifying a peptide, comprising a. producing a linker-peptide construct comprising a linker molecule and the peptide, wherein the peptide is covalently bonded to a synthesis solid phase and the linker molecule is covalently bonded to the peptide by the group R, b. separating the linker-peptide construct from the synthesis solid phase, c. binding the linker-peptide construct to a purification solid phase via the group A of the linker molecule, d. performing one or more washing steps, and e. releasing the peptide from the linker-peptide construct by splitting the group R of the linker molecule. The invention also relates to a kit for purifying a peptide using the method according to any of claims 6 to 13, characterised in that the kit comprises a. a linker molecule according to any of claims 1 to 5, or b. a linker molecule precursor comprising the groups R and L of a linker molecule according to any of claims 1 to 5 and reagents for the covalent bonding of the group A, optimally of the group A-E to the group R of the linker molecule precursor, and c. optionally reagents and solvents for the separation of protective groups of the group A of the linker molecule or the group R of the linker molecule precursor and for the one or more washing steps. The invention also relates to the use of the linker molecule in a method for purifying a peptide.