BioNukleo
BioNukleo GmbH is a specialist in sustainable enzymatic synthesis of nucleoside and nucleotide derivatives and related fine chemicals.
About
BioNukleo GmbH is a Berlin-based biotechnology company, founded in 2014 as a spin-off from the Technical University of Berlin. Specializing in the enzymatic synthesis of nucleoside and nucleotide derivatives as well as related fine chemicals, BioNukleo combines extensive expertise in biocatalysis and nucleoside chemistry to offer solutions that are both high in selectivity and sustainable. The company provides a wide range of well-characterized, in-house produced enzymes, process development services, and ready-to-use kits, supporting clients in drug development, synthetic biology, research, and diagnostics. With a strong commitment to research, innovation, and customer-centered solutions, BioNukleo aims to set new standards in sustainable chemical manufacturing.
Products & Services
- In-house enzyme library (nucleoside, nucleotide, and sugar modifying enzymes)
- Biocatalytic process development and custom synthesis
- Ready-to-use enzyme kits (e.g., PUB phosphate/pyrophosphate detection kits, Nucleoside2NTP Kits)
- Custom and catalog fine chemicals (pentose-1-phosphates, phosphate donors, nucleoside triphosphates)
- ATP regeneration enzyme systems
- Enzyme sets for nucleoside triphosphate synthesis
- Scientific consulting and process scale-up services
- Custom NTP synthesis
Use Cases & Case Studies
- Development of chromatography-free quantification methods for ribonucleotides in biological samples using BioNukleo kinases
- Synthesis of unnatural nucleoside triphosphates for research applications
- Facilitating green and efficient chemo-enzymatic synthesis for nucleoside/nucleotide analogues
- Providing animal-free and human-free enzymes for regulatory compliant bioprocessing
- Application in fine chemical and drug development research
Partnerships & Collaborations
- Collaboration with Technische Universität Berlin (Chair of Bioprocess Engineering)
- Member and board representation in NetPhaSol (Network for Pharma Solutions)
- Collaboration with Enzymicals in the field of enzymatic synthesis of nucleosides and nucleotides
- Partnership with abcr GmbH for the distribution of catalog products
- Joint project with TU Berlin (Department of Applied Biochemistry) for antiviral research
- Participation in Scientist.com as a registered supplier
Awards & Recognition
- Label "Innovative through research" from Stifterverband
- Co-founder and associates receiving poster awards and science prizes (e.g., Promotionspreis 2025 at TU Berlin)