Enzymes Catalyzing the Glycosylation of Polyphenols

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent discloses newly identified enzymes (specific glycosyltransferases) capable of catalyzing the glycosylation (attachment of sugar molecules) of polyphenols, especially classes such as flavonoids, benzoic acid derivatives, stilbenoids, chalconoids, chromones, and coumarin derivatives. The invention provides protein sequences, nucleic acids, and use cases for these enzymes to efficiently modify polyphenols, thus offering new tools for producing glycosylated polyphenols with enhanced properties.

Use CasesContent extracted from patent full text and abstract with AI.

• Production of glycosylated flavonoids and other polyphenols for supplements and nutraceuticals with improved solubility, stability, or bioavailability.
• Synthesis of modified polyphenols for pharmaceutical applications, such as improved drug formulations or new active compounds with better absorption.
• Generation of unique polyphenol derivatives for cosmetics to exploit their antioxidant, anti-inflammatory, or protective properties.
• Creation of novel food additives or functional foods with enhanced health benefits due to increased bioactivity or better taste profiles of glycosylated polyphenols.
• Biocatalysis in research settings for the preparation of glycosylated plant secondary metabolites for metabolic studies or biological assays.

BenefitsContent extracted from patent full text and abstract with AI.

• Allows regio- and stereo-specific glycosylation of a broad range of polyphenols, overcoming limits of chemical synthesis.
• Enables more efficient, scalable, and eco-friendly production of glycosylated polyphenols compared to traditional plant extraction or chemical modification methods.
• Improves the solubility, bioavailability, and stability of polyphenols, expanding their utility in pharmaceutical, food, and cosmetic industries.
• Facilitates the creation of new natural products and derivatives not readily found in nature, opening avenues for novel compounds with potentially superior therapeutic or nutraceutical properties.
• The enzymes are suitable for expression in microbial hosts, enabling industrial biotechnological processes.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The invention relates to enzymes catalyzing the glycosylation of polyphenols, in particular flavonoids, benzoic acid derivatives, stilbenoids, chalconoids, chromones, and coumarin derivatives. In one aspect the invention provides for an enzyme catalyzing the glycosylation of polyphenols,wherein the enzyme a) comprises an amino acid sequence according to one of the sequences of SEQ ID NO: 7-12, or b) is encoded by a nucleic acid comprising a nucleotide sequence of one of the sequences of SEQ ID NO: 1-6, or c) is homologous to one of the enzymes defined in a) or b) above, or d) is encoded by a nucleic acid hybridizing under stringent conditions with a nucleic acid complementary to a sequence comprising a nucleotide sequence of one of the sequences of SEQ ID NO: 1-6.