Transcriptional Synchronization of Two or More Functional Transcription Products

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a method and genetic construct for synchronizing the transcription of two or more functional gene products in a cell. By placing two genetic sequences under the control of the same promoter and regulatory elements, and using self-cleaving ribozymes to separate the resultant RNA products, both gene products are made in a controlled, matched, and simultaneous way. This enables precise linking of native gene functions to synthetic outputs such as proteins or RNA molecules.

Use CasesContent extracted from patent full text and abstract with AI.

• Creating disease-resistant plants or animals by coupling native defense genes with synthetic resistance RNAs or proteins.
• Developing safer and more precise gene therapies, where therapeutic gene products are only expressed when an endogenous gene is active.
• Engineering stem cells for effective tissue replacement and updating without undesired genetic mosaics.
• Simultaneously expressing a reporter gene for real-time monitoring of endogenous gene activity.
• Studying gene function in cells by directly linking knockdown or upregulation RNAs to the transcription of the genes of interest.
• Designing molecular 'logic circuits' in synthetic biology where outputs only occur if particular gene activity is present.
• Producing pathogen-resistant bacteria or crops with minimal impact on normal cell metabolism.
• Cancer therapy designs where therapeutic RNAs or proteins are only expressed when cancer-related gene transcription is dysregulated.

BenefitsContent extracted from patent full text and abstract with AI.

• Ensures output (e.g., therapeutic RNA/protein) is only produced when the target gene is active, reducing the risk of harmful overexpression.
• Minimizes metabolic burden on the host cell by restricting synthetic gene activity to relevant situations.
• Allows precise, stoichiometric matching of native and synthetic gene product levels in variable cellular states.
• Reduces genetic mosaicism in engineered tissues or organisms by linking output to essential cellular processes such as cell division or differentiation.
• Enables multi-layer gene regulation for safer and more adaptable synthetic biology or medical applications.
• Facilitates creation of "if-then" logical controls in living systems for precise biotechnological interventions.
• Improves reliability and predictability of synthetic gene circuits in living organisms.

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Patent Abstract

The invention relates to the transcriptional synchronization of two or more functional transcription products. In the invention a first functional transcription product and a second transcription product are placed under exactly the same transcriptional regime, i.e. the same transcription regulatory element(s) of a given transcription unit, in order to have both transcription products transcribed in a synchronized manner.