Methods for the Production of Rhamnosylated Flavonoids

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes novel methods for producing rhamnosylated flavonoids using specific glycosyl transferase enzymes. By expressing these enzymes (or using specialized host cells, like E. coli engineered to express them), flavonoids are selectively rhamnosylated at the 5-OH position—a site that is rarely modified in nature—resulting in new types of flavonoid-5-O-rhamnosides that can be produced reliably and at scale. The process overcomes previous limitations in flavonoid modification, allowing for high-yield, controllable production of these bioactive compounds with improved solubility and stability.

Use CasesContent extracted from patent full text and abstract with AI.

• Production of rhamnosylated flavonoids as ingredients for functional foods and dietary supplements.
• Development of cosmetic ingredients for anti-aging, skin firming, and hair growth applications, leveraging the enhanced bioactivity of rhamnosylated flavonoids.
• Creation of pharmaceutical compositions utilizing the antioxidant, anti-inflammatory, or anti-cancer properties of rhamnosylated flavonoids.
• Use in animal feed to enhance animal health by providing bioavailable antioxidant compounds.
• Scale-up manufacture of rare glycosylated flavonoid derivatives that are difficult or impossible to extract from natural sources.
• Research and development of improved drug delivery systems using the water-soluble nature of rhamnosylated flavonoids.

BenefitsContent extracted from patent full text and abstract with AI.

• Provides access to rare rhamnosylated flavonoids, especially 5-O-rhamnosides, which are nearly absent in nature and previously inaccessible in large quantities.
• Enables scalable, reliable industrial production (biotechnological/fermentation methods) that surpasses extraction from plant sources.
• Significantly improves water solubility and stability of flavonoids, broadening their usability in aqueous formulations.
• Rhamnosylation at the 5-O position can enhance bioavailability, activity, and reduce cytotoxicity, making flavonoids safer and more effective for use in health, nutrition, or cosmetic products.
• Allows customization: various flavonoid cores and derivative modifications can be produced by changing the substrate or the enzyme used.
• Facilitates research into novel bioactive compounds and their physiological effects due to easier access to diverse, previously rare structures.
• Enables production of cleaner, standardized compounds free from environmental contaminants often associated with plant extraction.

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Inventors & Applicants

Patent Abstract

The present invention relates to methods for the production of rhamnosylated flavonoids comprising the steps of contacting/incubating a glycosyl transferase with a flavonoid and obtaining a rhamnosylated flavonoid. In addition, the invention relates to glycosyl transferases suitable for use in such methods and kits comprising said glycosyl transferases.