Plant Regulatory Elements and Uses Thereof

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This invention relates to novel plant-derived DNA regulatory elements (such as promoters), DNA constructs, and their sequences that allow precise control and modification of gene expression in plants. These regulatory elements can be linked to genes of interest, facilitating targeted or tissue-specific expression of traits such as increased yield, improved stress tolerance, or enhanced nutritional content in genetically modified (transgenic) plants. The invention includes specific DNA sequences, methods for using them, and resulting transgenic plants, seeds, and commodity products.

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• Developing transgenic crops (like cassava, potato, or other staple foods) with improved yield, drought resistance, or nutrient content by enabling precise expression of beneficial genes.
• Engineering plants for resistance to pests, diseases, or environmental stresses by driving resistance gene expression in specific tissues or developmental stages.
• Producing biofortified foods with enhanced vitamins or minerals through targeted gene expression in edible plant parts.
• Generating high-starch or high-protein plants for industrial applications by enhancing relevant metabolic pathways in storage organs and tissues.
• Producing commodity products (such as protein isolates, starch, seed oils, flours, or meals) from genetically engineered plants that carry these regulatory elements and desired traits.
• Creating research tools for plant molecular biology by using promoters with specific expression profiles to investigate gene functions in various tissues or developmental stages.
• Stacking multiple transgenes in one plant without silencing, by leveraging a library of new, unique regulatory elements to control each gene independently.

BenefitsContent extracted from patent full text and abstract with AI.

• Provides a suite of well-characterized, novel plant promoters and regulatory elements for targeted genetic engineering, including tissue-specific and inducible expression in plants.
• Enables the expression of genes of agronomic interest with high precision, boosting effectiveness and minimizing unwanted side effects.
• Supports complex genetic modifications, such as polygenic trait improvement, by allowing independent control of multiple transgenes within one plant.
• Reduces the risk of transgene silencing, as the availability of diverse regulatory elements prevents repetitive sequence-related problems.
• Offers regulatory elements that can be tailored for specific crops, tissues, or desired timing, increasing flexibility and applicability across different plant species.
• Promotes sustainable agriculture by facilitating the development of crops with higher yield, improved resilience to climate change, and better nutritional quality, aiding food security.
• Allows for the generation of value-added plant products and processed goods with customized attributes for food, feed, and industrial uses.

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Patent Abstract

The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.