Method for Manufacturing L-Amino Acids

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent discloses a method to enhance the production of L-amino acids, particularly L-lysine, using genetically engineered coryneform bacteria. Specifically, it describes weakening the AmtR regulator gene in these bacteria, either by modifying its promoter region, substituting certain nucleotides or amino acids, or both. This weakening leads the bacteria to increase the synthesis and excretion of L-amino acids during fermentation processes. The invention also covers related recombinant bacteria, genetic constructs (polynucleotides, vectors), and methods for producing and isolating the amino acids.

Use CasesContent extracted from patent full text and abstract with AI.

• Industrial-scale production of amino acids like L-lysine, L-glutamic acid, L-glutamine, L-arginine, L-proline, and L-ornithine for food, pharmaceutical, and animal feed applications.
• Enhanced microbial fermentation for more cost-effective and efficient manufacture of amino acid-based supplements.
• Production of L-lysine-rich products for use in animal nutrition as feed additives to improve growth and health.
• Generation of improved bacterial strains for biotechnological research and commercial amino acid biosynthesis.

BenefitsContent extracted from patent full text and abstract with AI.

• Improved yields of L-amino acids during fermentation, boosting production efficiency and reducing manufacturing costs.
• Genetic modifications are precisely described, allowing targeted and stable strain improvement.
• Potential to adapt the approach for various amino acids central to multiple industries (food, pharma, feed).
• Ability to use standard fermentation techniques and equipment with improved microbial strains.
• Scalable process suitable for continuous or batch cultures, flexible production forms (liquid, powder, granulate), and streamlined downstream processing.
• Versatility in modifying various coryneform species and applicable to other industrial microbial hosts.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The object of the invention is a method for manufacturing L-amino acids using coryneform bacteria in which the AmtR regulator has been weakened, and corresponding recombinant bacteria, polynucleotides and vectors.