Device for investigation of a migration of adherent cells from a coating of a carrier coated by protein, comprises a barrier, which stands in contact with the carrier and is removable from the carrier

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a laboratory device for studying how adherent cells (cells that need a surface to grow) migrate after being prevented from occupying a specific area on a carrier coated with protein. The device uses a removable barrier to block cell growth in a defined area; once the barrier is taken away, researchers can observe cells migrating into the previously empty space. The device’s construction materials and methods ensure the barrier is easily attachable and removable without disturbing the cell culture.

Use CasesContent extracted from patent full text and abstract with AI.

• Research on wound healing by observing cell migration into a cleared area (analogous to a scratch assay).
• Testing the effects of drugs or chemicals on cell migration and invasiveness in cancer studies.
• Studying tissue regeneration and repair processes in vitro.
• Basic research in cell biology, including adhesion, motility, and response to different surface coatings.
• Assaying the effect of genetic modifications or treatments on cellular movement.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables precise and reproducible areas free from cells for studying cell migration.
• Removable barrier minimizes disturbance to the cell layer when initiating migration studies.
• Compatible with standard lab consumables such as petri dishes and microtiter plates.
• Facilitates high-throughput or parallel experiments for comparative analysis.
• Can be produced in various materials and sizes to accommodate multiple research needs.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The device (10) comprises a barrier (12), which stands in contact with the carrier and is removable from a carrier (25). The barrier prevents a growth of the cells on the carrier in the area of the contacts, so that an area kept free from the cells during the cultivation of cells on the carrier. The removal of the barrier permits the migration of the cells in the area. The barrier consists of a material on the area, which stands in contact with the carrier and the material non adheres on the coated carrier. The device (10) comprises a barrier (12), which stands in contact with the carrier and is removable from a carrier (25). The barrier prevents a growth of the cells on the carrier in the area of the contacts, so that an area kept free from the cells during the cultivation of cells on the carrier. The removal of the barrier permits the migration of the cells in the area. The barrier consists of a material on the area, which stands in contact with the carrier and the material non adheres on the coated carrier. The carrier is the base of the cell culture container or is arranged on the base of the cell culture container. The carrier is a plate made of glass or first plastic. The cellculture container is a Petri dish (11) or a dent of a microtiter plate made of second plastic. The barrier consists of third plastic and/or stainless steel. A means by which the barrier is pressed in the area of the contacts on the carrier is arranged on the barrier, the carrier or the cell culture container. The means is a spring (14) or clasp for clamping the barrier on the carrier or the cell culture container, formed from metals or fourth plastic or is a magnet. The spring and the barrier or the clasp and the barrier are formed in single piece manner. The barrier is produced in injection molding method. The barrier has coating made of further protein on the area, which stands in contact with the carrier. The surface of the barrier is functionalized through physical or chemical modification of the surface or through applying of inert coating. The carrier and the barrier or the cell culture container and the barrier comprise plane surface corresponding in the area of the contacts in its form. The barrier is formed so that the area free from cells has width of 0.5-2 mm or diameter of 0.5-2 mm. The barrier is formed so that the area free from cells is widened on both ends of the strip. An independent claim is included for a method for investigation of a migration of adherent cells from a coating of a carrier coated by protein.