Device for the Production and/or Purification of Nanoparticle-Coupled Biomaterials and Method Using the Same

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This patent describes a device and method for the gentle production and/or purification of nanoparticle-bound biomaterials, such as proteins or DNA attached to nanoparticles. The device includes a chamber with a sidewall opening fitted with a filter, positioned so that an end volume remains below the filter after liquid passes through. The method uses filtration, often assisted by vacuum or fluid flow, to separate larger particles (like nanoparticles or cells) from smaller molecules, without applying damaging centrifugal forces. This results in a faster and milder purification process compared to conventional centrifugation-based techniques.

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• Preparation and purification of nanoparticle-bioconjugates (e.g., antibodies, proteins, DNA linked to nanoparticles) for biomedical applications
• Purification of therapeutic antibodies and removal of aggregates before clinical or laboratory use
• Gentle separation of cells, viruses, or bacteria from biological fluids (e.g., blood, urine, cerebrospinal fluid)
• Production of SERS (surface-enhanced Raman scattering) active complexes for analytical diagnostics or research
• Removal of low molecular weight stabilizers and unreacted chemicals from nanoparticle formulations
• Purification of proteins and removal of contaminating chemicals or stabilizers (e.g., BSA, gelatin, sodium azide)
• Sample preparation in nanotechnology- or biotechnology-related research and diagnostics

BenefitsContent extracted from patent full text and abstract with AI.

• Significant reduction in processing time compared to centrifugation (up to 95% faster)
• Minimized damage to sensitive biomolecules (e.g., proteins, antibodies) and nanoparticles due to the gentle filtration process
• Prevents aggregation or degradation of biomolecules by avoiding high shear forces
• Flexible and customizable according to filter pore size and chamber design, suitable for a wide range of particles and biomolecules
• Can be scaled and used for both laboratory and clinical sample preparation needs
• Supports high purity and activity of sensitive biomaterials, enhancing the reliability and reproducibility of downstream diagnostics or therapies
• Reduces risk of contamination and loss compared to traditional separation techniques

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Patent Abstract

Vorrichtung, umfassend eine Kammer mit zumindest einer Öffnung, wobei die Öffnung mit einem Filter verschlossen ist und wobei die Öffnung in einer der Seitenwände der Kammer derart angeordnet ist, dass zwischen der Öffnung und dem Boden der Kammer ein Endvolumen gebildet ist.