Lipid analysis

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention provides a highly sensitive, non-radioactive method for detecting and analyzing lipids based on click chemistry. Specifically, it involves reacting alkyne-modified lipids with azido-modified coumarin compounds to generate a fluorescent product that can be easily separated and detected using chromatography and fluorescence imaging. The technology covers new synthetic routes for preparing these alkyne-lipid derivatives, novel fluorescent adducts, and their use in assays for lipid metabolism, enzyme activity, and biological research.

Use CasesContent extracted from patent full text and abstract with AI.

• Studying lipid metabolism in living cells, tissues, or organisms across a wide range of biological systems (e.g., mammalian cells, bacteria, yeast, plants, insects).
• High-throughput lipid analysis in drug discovery or biomedical research.
• Monitoring fatty acid, cholesterol, and sphingolipid metabolism to understand diseases related to metabolic overload, such as obesity and diabetes.
• Enzyme activity assays for lipid-processing enzymes (e.g. lipases, transferases, phospholipases).
• Pulse-chase metabolic experiments to track lipid incorporation and turnover.
• Development of non-radioactive assay kits for academic, clinical, or pharmaceutical laboratories.
• Visualization of lipid localization or spatial distribution using microscopic imaging of fluorescence-labeled lipids.

BenefitsContent extracted from patent full text and abstract with AI.

• Eliminates the need for radioactive labeling, reducing health, regulatory, and disposal concerns.
• Provides higher sensitivity and lower detection limits than traditional radiolabeling methods.
• Enables linear, quantitative, and rapid measurements: analysis can be completed within a day.
• Compatible with a wide range of lipid classes and biological samples (universally applicable).
• Can be scaled down for high-throughput formats (e.g., 96-well plate assays), saving resources and increasing experimental flexibility.
• Produces fluorescent lipid conjugates that allow direct, real-time imaging and quantification.
• Greater safety, lower costs, and improved versatility over previous analytical techniques.
• Facilitates combination with other methods such as mass spectrometry or light microscopy.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The present invention relates to a method for the detection of a lipid comprising reacting an alkyne-modified lipid derivative and an azido-modified coumarin compound, purifying the resulting compound using chromatography and detecting the reaction product. In a further aspect the present invention provides new alkyne lipid derivatives, new methods of alkyne lipid synthesis, new uses of such compounds and new methods employing these compounds.