Method for Isolating Neural Cells Using Tenascin-R Compounds

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention provides a method for isolating neural cells, particularly oligodendrocytes, from primary neural tissue using tenascin-R (TN-R) compounds, including full proteins, fragments, and fusion proteins. By coating a substrate with TN-R or its fragments, specific neural cell types adhere and can be isolated in a single step, resulting in highly pure cell populations. The invention also describes recombinant production of these TN-R compounds, the use of antibodies for detection and isolation, and kits to facilitate these processes. The method significantly simplifies and speeds up the isolation of defined neural cell populations compared to traditional methods.

Use CasesContent extracted from patent full text and abstract with AI.

• Isolation of pure oligodendrocyte populations from neural tissues for research or therapeutic purposes.
• Production of differentiated neural cells for neurobiological, cell physiological, and clinical research.
• Development of diagnostic and therapeutic procedures for neurodegenerative diseases, including multiple sclerosis (MS) and periventricular leukomalacia.
• Screening and testing of drug candidates targeting neural cells, particularly in demyelinating conditions.
• Development of cell therapies for diseases involving loss of oligodendrocytes or myelin.
• Purification of neural stem/progenitor cells for transplantation or experimental studies.
• Generation of kits for routine cell isolation procedures in laboratories.
• Use in the manufacture of antibodies for TN-R detection and isolation purposes.

BenefitsContent extracted from patent full text and abstract with AI.

• Highly efficient, fast, and one-step isolation procedure for neural cells, especially oligodendrocytes, compared to existing multi-step and time-consuming methods.
• Produces highly purified neural cell populations, enabling more accurate research and applications.
• Applicable across a range of vertebrate species and neural tissues at various development stages.
• Minimizes cell loss and increases cell yield from primary tissue samples.
• Facilitates downstream applications such as differentiation, therapeutic testing, and diagnostics.
• Can be implemented using recombinant TN-R or specific fragments, enhancing reliability, reproducibility, and scalability.
• Offers the potential for automated, kit-based workflows, streamlining laboratory processes.
• Provides tools (antibodies, vectors, recombinant proteins) for broader research and clinical utility, including influencing neural development and nerve injury repair.

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Patent Abstract

The invention relates to a method for isolating neural cells using tenascin-R compounds, to tenascin-R fragments that are especially useful for said method and to tenascin-R fusion proteins. The invention also relates to the recombinant production of said tenascin-R compounds, to a kit for carrying out said method and to the use of said method for producing highly purified neural cell populations, and to antibodies that are suitable for the detection and isolation of tenascin-R compounds.