High-resolution transcriptome of human macrophages

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent provides a method to identify, characterize, and isolate two distinct types of human macrophages—M1-like (classically activated) and M2-like (alternatively activated)—by discovering and utilizing specific cell surface marker molecules unique to each type. Advanced transcriptome analysis (including RNA-seq) revealed markers such as CD120b, TLR2, and SLAMF7 for M1-like macrophages, and CD1a, CD1b, CD93, and CD226 for M2-like macrophages. The invention also includes kits, reagents, and protocols for using these markers to separate and study the two macrophage types.

Use CasesContent extracted from patent full text and abstract with AI.

• Clinical and biomedical research to study immune responses by isolating and analyzing specific macrophage populations.
• Development of diagnostic tools to assess macrophage polarization in disease states including infections, autoimmune disorders, and cancer.
• Drug development and screening, enabling specific targeting of M1-like or M2-like macrophages for therapeutic interventions.
• Transplant monitoring through identification of inflammatory (M1) versus tissue-repair (M2) macrophages in biopsies.
• Cell therapy, allowing enriched populations of specific macrophage types for regenerative or immunomodulatory applications.

BenefitsContent extracted from patent full text and abstract with AI.

• Facilitates precise identification and separation of macrophage subtypes based on unique surface markers, improving research accuracy.
• Enables high-resolution analysis of macrophage function and polarization, advancing understanding of immune regulation in health and disease.
• Supports the development of more targeted and effective diagnostics and therapies by distinguishing between pro-inflammatory and anti-inflammatory macrophage types.
• Improves the reproducibility and efficiency of macrophage-related studies via validated, marker-based protocols and kits.
• Opens opportunities for personalized medicine approaches by linking macrophage polarization states with patient responses or prognoses.

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Inventors & Applicants

Patent Abstract

The invention is based on the finding of specific surface markers for M1-like (classically activated) and M2-like (alternatively activated) macrophages and provides for a method for the identification, characterization and isolation of M1-like and M2-like macrophages based on the abundance of said surface markers and for means for performing such method.