Up-scaled Production of Microglia-Like/-Precursor Cells and Macrophage Cells Using Mesh Macrocarriers

Simple SummaryContent extracted from patent full text and abstract with AI.

This invention presents a scalable method for producing large quantities of microglia-like, microglial precursor, and macrophage cells from embryoid bodies (EBs) by using specially designed mesh macrocarriers. These porous, coated mesh structures support cell adherence and 3D outgrowth in bioreactor systems, enabling the generation and easy harvesting of pure cell populations. The produced cells can also be efficiently cryopreserved for later use, addressing prior limitations in cell yield, quality, and scalability in the production of these important immune cell types.

Use CasesContent extracted from patent full text and abstract with AI.

• Manufacture of large quantities of human microglia-like or macrophage cells for neurological disease research (e.g. Alzheimer’s, Parkinson’s, multiple sclerosis)
• Drug screening and toxicity testing using physiologically relevant cellular models
• Cell therapy or regenerative medicine research for neurodegenerative and inflammatory disorders
• Basic research into immune cell development and function in the central nervous system
• Bio-banking of cryopreserved microglia-like and macrophage cells for later distribution and use
• Production of disease-specific or patient-specific cell lines from induced pluripotent stem cells

BenefitsContent extracted from patent full text and abstract with AI.

• Enables industrial-scale and reproducible production of functionally relevant microglia-like and macrophage cells
• Reduces costs and labor compared to traditional 2D culture techniques
• Improves cell yield and purity, making studies and applications more efficient
• Cells are highly suitable for cryopreservation, maintaining viability and function after thawing
• Harvesting is simplified due to floating macrocarrier design and dynamic bioreactor culture
• Supports the production of cells from human iPSCs, including patient-derived lines for personalized medicine
• Provides a standardized and scalable platform for research and development in neuroscience and immunology

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Inventors & Applicants

Patent Abstract

The present invention relates to methods allowing adherence and outgrowth of embryoid bodies (EBs) using macrocarriers. The methods of the invention are useful for an up-scaled production of myeloid cells, such as macrophage- and microglia-like/-precursor cells, in a bioreactor system. The invention further relates to microglia-like cells or microglial precursor cells obtainable by these methods that are cryopreservable. The invention also concerns a porous macrocarrier coated with a material facilitating cell adherence.