Induced Neural Stem Cells

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a novel method for generating induced neural stem cells (iNSCs) by directly reprogramming somatic cells (like fibroblasts) using the Oct4 protein (or a similar variant) for a controlled, limited period. Unlike earlier techniques that require creating pluripotent stem cells (which can form tumors), this approach directly converts cells into neural stem cells, which are expandable, stable, and able to differentiate into the three main types of neural cells: neurons, astrocytes, and oligodendrocytes. The method can be performed with various delivery approaches (protein, mRNA, viral vectors, inducible expression) and is suitable for use with human cells.

Use CasesContent extracted from patent full text and abstract with AI.

• Production of patient-specific neural stem cells for regenerative therapies in neurological diseases (such as Parkinson's, Alzheimer's, or spinal cord injury).
• In vitro disease modeling and drug screening using patient-derived neural stem cells.
• Generation of various neural cell types for basic research into brain development and neurodegenerative disease mechanisms.
• Potential treatment for injuries or degenerative diseases by transplanting induced neural stem cells or their derivatives into patients.
• Studying genetic neurological disorders using isogenic neural cells derived from patients' own somatic cells.

BenefitsContent extracted from patent full text and abstract with AI.

• Enables direct and efficient conversion of somatic cells to neural stem cells without passing through a pluripotent stage, reducing tumorigenic risk.
• Patient-specific iNSCs can minimize immune rejection issues in transplantation.
• Produced iNSCs are stably expandable (can be cultured and grown for many passages), enabling reliable manufacturing and research use.
• iNSCs generated by this method can differentiate into all major neural lineages (neurons, astrocytes, oligodendrocytes), increasing their utility.
• The method allows for temporal control over reprogramming factors, enhancing safety and specificity.
• Avoids lengthy, complicated procedures required for traditional iPSC-based neural cell generation.
• Applicable to human cells, broadening its clinical and research impact.

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Patent Abstract

The present invention relates to a method of deriving an induced neural stem cell (iNCS) by nuclear reprogramming of a somatic cell, wherein the method comprises a step of contacting the somatic cell with Oct4 protein or a functionally equivalent analogue, variant or fragment thereof for a limited time period, as well as an induced neural stem cell obtained by this method.