Purification of triphosphorylated oligonucleotides using capture tags

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes a new method for synthesizing and purifying oligonucleotides that are modified with a triphosphate group at the 5'-end, using a special 'capture tag.' The process allows for highly efficient purification, enabling the production of oligonucleotides with very high yield and purity that are suitable for pharmaceutical use. The capture tag temporarily attaches to the oligonucleotide, greatly simplifying the separation of the desired triphosphorylated product from impurities. The tag can be removed after purification or left on if useful for further applications.

Use CasesContent extracted from patent full text and abstract with AI.

• Pharmaceutical development of synthetic RNA drugs and vaccine adjuvants that require high-purity triphosphorylated oligonucleotides.
• Production of RNA molecules for immunotherapy, such as RIG-I agonists for cancer or infectious disease treatment.
• Research applications needing clean, high-purity triphosphorylated oligonucleotides, such as studies on RNA interference (RNAi) or gene regulation.
• Manufacture of oligonucleotide standards for diagnostics involving triphosphorylated nucleic acids.
• Development of kits and reagents for custom synthesis and purification of triphosphorylated oligonucleotides in laboratory and industry settings.

BenefitsContent extracted from patent full text and abstract with AI.

• Delivers high purity and yield of triphosphorylated oligonucleotides suitable for pharmaceutical and clinical applications.
• Greatly simplifies the purification process compared to traditional methods (e.g., avoids limitations of gel or ion exchange chromatography).
• Reduces contamination with similar or incomplete sequences (n-1, n-2, and mono-/diphosphates), improving product quality.
• Compatible with a wide variety of oligonucleotide sequences and chemical modifications, making the method broadly applicable.
• Enables production at scales appropriate for preclinical and clinical studies, supporting drug development and commercialization.

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Inventors & Applicants

Patent Abstract

The present invention relates to a method of preparing triphosphate-modified oligonucleotides using a capture tag. The method allows the synthesis and purification of triphosphate-modified oligonucleotides in high yield and purity suitable for pharmaceutical applications.