Use of Cellobiofructose and Production Method Thereof

Simple SummaryContent extracted from patent full text and abstract with AI.

This patent describes the use of cellobiofructose (CBF), a novel trisaccharide, as a dietary fiber and prebiotic in foods, beverages, nutraceuticals, and pharmaceuticals. It details both the health benefits of CBF—such as promoting beneficial gut bacteria and not raising blood sugar—and an improved, efficient biotechnological method for producing high-purity CBF using engineered host microorganisms expressing levansucrase. CBF is highly soluble, can replace sugar as a low-calorie sweetener, and supports the growth of probiotic bacteria.

Use CasesContent extracted from patent full text and abstract with AI.

• Functional food and beverage formulations (such as yogurts, smoothies, juices, dairy drinks) seeking fiber enrichment and prebiotic benefits.
• Production of reduced-sugar or sugar-free products (bakery, confections, syrups, sauces, ice creams) where sweetness, texture, and fiber are important.
• Development of nutraceuticals and dietary supplements targeting gut health, metabolic syndrome, or cardiometabolic diseases.
• Formulation of meal replacements or nutrition shakes with added prebiotic fiber.
• Pharmaceutical compositions for supportive management of obesity, constipation, high cholesterol, diabetes, hypertension, and heart disease.
• Animal feeds to promote a beneficial gut microbiota and health in livestock or pets.

BenefitsContent extracted from patent full text and abstract with AI.

• Provides both dietary fiber and prebiotic effects, supporting beneficial gut microbiota such as bifidobacteria and lactobacilli.
• Does not raise blood glucose levels, making it suitable for diabetics and people on low-glycemic diets.
• Highly soluble, allowing easy incorporation into diverse products without affecting texture or clarity.
• Acts as a natural low-calorie sweetener, enabling sugar reduction while maintaining desirable mouthfeel and taste.
• Promotes satiety and aids digestive health due to fiber properties (can help prevent constipation).
• Safe (non-cytotoxic) and readily degradable by human gut microbiota and environmental microbes, minimizing accumulation and environmental impact.
• Efficient biotechnological production method improves yield and purity, reducing production costs and complexity compared to conventional methods.
• Stable under various food processing conditions and suitable for a broad spectrum of applications.

Technical Classifications (CPCs)

Inventors & Applicants

Patent Abstract

The present invention relates to the use of cellobiofructose (CBF) as a dietary fiber and/or prebiotic component in food products, food product additives, nutraceuticals and/or beverages or for increasing the growth of probiotic microorganisms. The invention further relates to CBF and compositions containing it for use in the prevention and/or treatment of obesity, constipation, high cholesterol, diabetes, hypertension, and/or heart disease. The invention further encompasses a method for the (recombinant) production of cellobiofructose (CBF), as described herein, using a polypeptide having levansucrase activity which comprises or consists of a polypeptide having at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 1.